The Biomedical Research Centre

Journal Articles

Aebersold, R., Bures, E.J., Namchuk M, Goghari M.N., Sushan, B., and Covey, T.C. Design, synthesis and characterization of a protein sequencing reagent yielding amino acid derivatives with enhanced detectability by mass spectrometry. Protein Science, 1: 494-503, 1993.

Baraban, J.M., Fiore, R.S., Sanghera, J.S., Paddon, H.B., and Pelech, S.L. Identification of p42 MAP kinase as a tyrosine kinase substrate activated by electroconvulsive treatment in hippocampus. J. Neurochem., 60:330-336, 1993.

Carter, J.R., Franden, M.A., Aebersold, R., and McHenry, C.S. Identification, isolation and characterization of the structural gene encoding the d' subunit of E coli DNA polymerase III holoenzyme. J. Bacteriol., 175:3812-3827, 1993.

Carter, J.R., Franden, M.A., Aebersold, R., and McHenry, C.S. Identification, isolation and overexpression of the gene encoding the y subunit of DNA polymerase III holoenzyme. J. Bacteriol., 175:5604-5610,
1993.

Carter, J.R., Franden, M.A., Aebersold, R., Kim, D.R., and McHenry, C.S. Isolation, sequencing and overexpression of the gene encoding the X subunit of E. coli DNA polymerase III holoenzyme. Nucl. Acids Res., 21:3281-3286, 1993.

Charest, D.L., Mordret, G., Harder, K.W., Jirik, F.R., and Pelech, S.L. Molecular cloning, characterization and expression of human p43ERK1, a tyrosyl-phosphorylated and activated protein-seryl/theronyl kinase. Mol. Cell. Biol., 13:4679-4690, 1993.

Clark-Lewis, I., Dewald, B., Geiser, T., Moser, B., and Baggiolini, M. Platelet factor 4 binds interleukin-8 receptors and activates neutrophils when the N-terminus is modified with Glu-Leu-Arg. Proc. Natl. Acad. Sci. USA, 90:3574-3577, 1993.

Crawford, T.J., Melhado, I., and Jirik, F.R. Expression of versican mRNA is developmentally regulated in the brain of the embryonic chick and developing rat. Develop. Brain Res., 76:264-267, 1993.

Daga, A., Mical, V., Hess, D., Aebersold, R., and Attardi, G. Molecular characterization of the transcription termination factor (mTERF) from human mitochondria. J. Biol. Chem., 268:8123-8130, 1993.

Daya-Makin, M., Szankasi, P., Tang, MacRae, D., and Pelech, S.L. Regulation of p105wee1 and p34cdc2 during meiosis in Schizosaccharomyces pombe. Biochem. Cell Biol., 70:1088-1096, 1993.

Federsppiel, B., Duncan, A.M.V., Delaney, A., Schappert, K., Miki, N., Clark-Lewis, I., and Jirik, F.R. Molecular cloning of the cDNA chromosomal localization of the gene, and expression pattern of a putative seven transmembrane segment (7-TMS) receptor isolated from human spleen. Genomics, 16:707-712, 1993.

Fiore, R.S., Murphy, T.H., Sanghera, J.S., Pelech, S.L., and Baraban, J.M. Activation of p42 MAP kinase by glutamate receptor stimulation in rat primary cortical cultures. J. Neurochem., 61: 1-8, 1993.

Geiser, T., Dewald, B., Ehrengruber, M.V., Clark-Lewis, I., and Baggiolini, M. The interleukin 8 related chemotactic cytokines GROa, GROb and GROg activate human neutrophil and basophil leukocytes. J. Biol. Chem., 268:15419-15424, 1993.

Harrington, M.G., Aebersold, R., Martin, B.O., Merrill, C.R., and Hood, L.E. Identification of a brain-specific cerebrospinal fluid glycoprotein beta-trace protein. Appl. and Theor. Electrophoresis, 3:229-234, 1993.

Hei, Y.-J., Pelech, S.L., Diamond, J., and McNeill, J.H. Insulin-stimulated seryl/threonyl protein kinases in diabetic rats and the effects of vanadium treatment. In: NATO ASI Series Vol. H 76, Tyrosine Phosphorylation/Dephosphorylation and Downstream Signalling, ed. Heilmeyer, L., Springer-Verlag, Berlin, pp 227-230, 1993.

Henderson, H.E., Ma, Y., Lui, M.S., Clark-Lewis, I., Maeder, D.L., Kastelein, J.J.P., Brunzell, J.D., and Hayden, M.R. Structure-function relationships of lipoprotein lipase: Mutation analysis and mutagenesis of the loop region. J. Lipid Res., 34:1593-1602, 1993.

Hess, D., Covey, T.C., Winz, R., Brownsey, R., and Aebersold, R. Analytical and micropreparative peptide mapping by high-performance liquid chromatography/ion spray mass spectrometry of proteins purified by gel electrophoresis. Protein Science, 2:1342-1351, 1993.

Kabir, S., Aebersold, R., and Daar, A.S. Identification of a novel 4kDa immunoglobulin A binding peptide obtained after limited proteolysis of jacalin. Biochem. Biophys. Acta., 1161:194-200, 1993.

Keller, S., Aebersold, R., Garner, C., and Lienhard, G.E. The insulin-elicited 160kDa phosphotyrosine protein in mouse adipocytes is an insulin receptor substrate 1: Identification and cloning. Biochem. Biophys. Acta., 1172:323-326, 1993.

Klingemann, H.-G., Neerunjun, J., Schwulera, U., and Ziltener, H.J. Culture of normal and leukemic bone marrow in interleukin-2: Analysis of cell activation, cell proliferation and cytokine production. Leukemia, 7:1389-1393, 1993.

Li, X., Rock, F., Aarden, L., Chong, P., Cockle, S., Keating, A., Ziltener, H.J., and Klein, M. Structure-function analysis of the C-terminal segment of human interleukin-6. J. Biol. Chem., 268:22377-22384, 1993.

Moser, B., Dewald, B., Barella L., Schumacher C., Baggiolini, M., and Clark-Lewis, I. Interleukin-8 antagonists generated by N-terminal modification. J. Biol. Chem., 268:7125-7128, 1993.

Ophir, A., Berenshtein, E., Ziltener, H.J., and Razin, E. 5-Fluorouracil and mast cell precursor in mice. Exp. Hematol., 21:1558-1562, 1993.

Pelech, S.L. Networking with protein kinases. Current Biology, 3:513-515, 1993.

Pelech, S.L. The malignant connection: When its molecular 'nervous system' goes awry, a cell can turn cancerous. The Sciences (N. Y. Academy of Sciences), 33:32-36, 1993.

Pelech, S.L. and Sanghera, J.S. Mitogen-activated protein kinases, versatile transducers in cell signalling. Trends. Biochem. Sci., 17:233-238, 1993.

Pelech, S.L., Charest, D.L., Mordret, G.P., Siow, Y.L., Palaty, C., Campbell, D., Charlton, L., Samiei, M., and Sanghera, J.S. Networking with mitogen-activated protein kinases. Mol. Cell. Biochem., 127/128:157-170, 1993.

Peppel, K., Poltorak, A., Melhado, I., Jirik, F., and Beutler, B. Expression of a TNF inhibitor in transgenic mice. J. Immunol., 151:5699-5703, 1993.

Peter, M., Sanghera, J. S., Pelech, S. L., and Nigg, E. Mitogen-activated protein kinases phosphorylate nuclear lamins and display sequence specificity overlapping that of mitotic protein kinase p34cdc2. Eur. J. Biochem., 205: 287-294, 1993.

Pownall, S., Kozak, C.A., Schuppert, K., Sakar, M., Hull, E., Schachter, H., and Marth, J.D. Molecular cloning and characterization of the mouse UDP-N-acetylglucosamine: a-3-D-mannoside b-1,2- -acetylglucosaminyltransferase I gene. Genomics, 12:699-704, 1993.

Ramachandran, C., Aebersold, R., Tonks, N.K, and Pot, D.A. Sequential dephosphorylation of a multiply phosphorylated insulin receptor peptide by protein tyrosine phosphatases. Biochem., 31: 4232-4238, 1993.

Rosenshine, I., Duronio, V. and Finlay, B.B. Tyrosine protein kinase inhibitors block invasin-promoted bacterial uptake by epithelial cells. Infection and Immunity, 60: 2211-2217, 1993.

Samiei, M., Sanghera, J.S., and Pelech, S.L. Activation of myelin basic protein and S6 peptide kinases in phorbol ester -and PAF-treated sheep platelets. Biochim. Biophys. Acta., 1176(3):287-98, 1993.

Sanghera, J. S., Charlton, L., Paddon, H. B., & Pelech, S. L. Purification and characterisation of casein kinase II from sea star oocytes. Biochem. J., 283: 829-837, 1993.

Sanghera, J.S., Hall, F.L., Warburton, D., Campbell, D. and Pelech, S.L. Thr-669 phosphorylation site peptide from epidermal growth factor receptor is phosphorylated by distinct MAP kinase isoforms. Biochim. Biophys. Acta, 1135: 335-342, 1993.

Sanghera, J.S., Peter, M., Nigg, E.A. and Pelech S.L. Immunological characterization of MAP kinases in chicken DU249 cells: Evidence for nuclear localization. Molecular Biology of the Cell, 3: 775-787, 1993.

Schindler, C., Fu, X.Y., Improta, T., Aebersold, R., and Darnell, J.E. Jr. The proteins of ISGF-3: One gene encodes 91 and 84kDa ISGF-3a proteins. Proc. Natl. Acad. Sci USA, 89: 7836-7839, 1993.

Schrader, J.W. Biological effects on myeloid growth factors. Baillière's Clinical Hematology, eds. Lord, B.I. and Dexter, T.M., Baillière Tindall, WB Saunders Co., pp.509-532, 1993.

Schrader, J.W. Colony Stimulating Factors. Encyclopedia of Immunology, eds. Roitt, I.M. and Delves, P.J., Academic Press, London, pp. 369-370, 1993.

Schrader, J.W. Interleukin-3 - Its Structure and Function. Encyclopedia of Immunology, eds. Roitt, I.M, and Delves, P.J. Academic Press Ltd. London, pp 908-910, 1993.

Schrader, J.W. Re-examining the autostimulatory loop in acute myeloid leukemia. Advances in Cancer Therapy 4, Mediscript, London, U.K., pp. 5-16, 1993.

Schrader, J.W., Clark-Lewis I., Leslie K.B. and Ziltener, H.J. Interleukin-3. Handbook of Cytokines, eds. Aggarwal, B.B. and Gutterman, J.V., Blackwell Scientif. Publicat. Ltd., Cambridge MA, pp. 97-112 (1993).

Schumacher, C., Clark-Lewis, I., Baggiolini, M., and Moser, B. High and low affinity binding of GROa and neutrophil activating peptide-2 to interleukin 8 receptors on human neutrophils. Proc. Natl. Acad. Sci. USA, 89: 10542-10546 (1993).

Shi L, Kraut R, Aebersold R, and Greenberg A. A natural killer (NK) cell granule protein that induces DNA fragmentation and apoptosis. J. Exp. Med., 175: 553-566 (1993).

Smith, W.B., Gamble, J.R., Clark-Lewis, I., and Vadas, M.A. Chemotactic desensitization of neutrophils demonstrates interleukin-8 (IL-8) - dependent and IL-8-independent mechanisms of transmigration through cytokine-activated endothelium. Immunology, 78:491-497 (1993).

Tao, W., Teh, S.-J., Melhado, I.G., Jirik, F., Korsmeyer, S., and Teh, H.-S. The BCL-2 proto-oncogene participates in T cell development and repertoire selection. J. Exp. Med., 179: 145-153 (1993).

Tighe, H., Chen, P.P., Tucker, R., Kipps, T.J., Rovdier, J., Jirik, F.R., and Carson, D.A. Function of B-cells expressing a human IgM rheumatoid factor autoantibody in transgenic mice. J. Exp. Med., 177: 109-118 (1993).

Tomlinson-Jones, A., and Ziltener, H.J. Enhancement of the biologic effects of IL-3 in vivo by anti-IL-3 antibodies. Blood, 82:1133-1141 (1993).

Van Oers, N.S.C., Tao, W., Watts, J.D., Johnson, P., Aebersold, R., and Teh, H.-S. Constitutive tyrosine phosphorylation of the T-cell receptor (TCR) z subunit: Regulation of TCR associated tyrosine kinase activity by TCR z. Molec. Cell. Biol., 13:5771-5780 (1993).

Veals, S.A., Fu, X.Y., Leonard, D., Schindler, A., Aebersold, R., Darnell, J.E., and Levy, D.E. Subunit of an IFNa responsive transcription factor is related to interferon regulatory factor and Myb families of DNA binding proteins. Molec. Cell. Biol., 12: 3315-3324 (1993).

Vogt, T., Aebersold, R., and Ellis, B. Purification and characterization of Sinapine synthase from seeds Brassica napus. Arch. Biochem. Biophys., 300:622-628 (1993).

Wallace C.J.A. and Clark-Lewis, I. Functional role of heme ligation in cytochrome C: Effects of replacement of methionine 80 with natural and non-natural residues by semisynthesis. J. Biol. Chem., 267: 3852-3861 (1993).

Walz, A., Meloni, F., Zwahlen, R., Clark-Lewis, I., and Car, B. Generation and properties of neutrophil activating peptide 2 (NAP-2). Host Defense Dysfunction in Trauma, Shock and Sepsis, eds. Faist, Meakins, and Schildberg, Springer-Verlag, Berlin, pp. 593-598 (1993).

Watts, J.D., Sanghera, J.S., Pelech, S., and Aebersold, R. Serine 59 of p56lck is phosphorylated in vitro by a mitogen activated protein kinase and in vivo in activated T cells. J. Biol. Chem., 268:23275-23282 (1993).

Watts, J.D., Welham, M.J., Kalt, L., Schrader, J.W., and Aebersold, R. IL-2 stimulation of T lymphocytes induces sequential activation of mitogen- activated protein kinases and phosphorylation of p56lck at serine-59. J. Immunol., 151:6862-6871 (1993).

Watts, J.D., Wilson, G.M., Ettehadieh, E., Clark-Lewis, I., Kubanek, C.A., Astell, C.R., Marth, J.D. and Aebersold, R.A. Purification and characterization of the lymphocyte specific protein tyrosyl kinase p56lck
from a baculovirus expression system. J. Biol. Chem., 267:901-907 (1993).

Webb, L.M.C., Ehrengruber, M.V., Clark-Lewis, I., Baggiolini, M., and Rot, A. Binding to Heparan sulphate or heparin enhances neutrophil responses to interleukin-8. Proc. Natl. Acad. Sci. USA, 90:7158-7162 (1993).

Weinstein, S.L., Sanghera, J.S., Lemke, K., DeFranco, A.L. and Pelech, S.L. Bacterial lipopolysaccharides induces tyrosine phosphorylation and activation of MAP kinases in macrophages. J. Biol. Chem., 267:14955-14962 (1993).

Welham, M. and Schrader, J.W. Steel factor induced tyrosine phosphorylation in murine mast cells: Common elements with IL-3 induced signal transduction pathways. J. Immunol., 149:2772-2783 (1993).

Welham, M.J., Duronio, V., Sanghera, J.S., Pelech, S.L. and Schrader, J.W. Multiple hemopoietic growth factors stimulate activation of MAP kinase family members. J. Immunol., 149:1683-1693 (1993).

Williams, N.G., Paradis, H., Agarwal, S., Charest, D.L., Pelech, S.L., and Roberts, T.M. Raf-1 and p21v-ras cooperate in the activation of MAP kinase. Proc. Natl. Acad. Sci. USA, 90(12):5772-6 (1993).

Williams,R., Sanghera, J., Wu, F., Carbonaro-Hall, D., Warburton, D., Campbell, D., Pelech, S.L., and Hall, F. Identification of a human EGF receptor associated protein kinase as a new member of the MAPK/ERK family. J. Biol. Chem., 268:18213-18217 (1993).

Wong, Q., Tull, D., Meinke, A., Gilkes, N.R., Warren, R.A.J., Aebersold, R., and Withers, S.G. Glu 280 is the nucleophile in the active site of clostridium thermocellum Cel C, a family A endo-b-1,4 glucanase. J. Biol. Chem., 268:14096-14102 (1993).

Wu, C.B., Pelech, S.L. and Veis, A. The in vitro phosphorylation of the native rat incisor dentin phosphophoryns. J. Biol. Chem., 267:16588-16594 (1993).

Ziltener, H.J. Glycosylation does not affect in vitro biological activity of interleukin-3. Cytokine, 5:291-297 (1993).

Ziltener, H.J., Maines-Bandiera, S., Schrader, J.W., and Auersperg, N. Secretion of IL-1, IL-6, and colony-stimulating factors by human ovarian surface epithelium. Biol. Reprod., 49:635-641 (1993).

Books and Book Chapters

Schrader, J.W. Re-examining the autostimulatory loop in acute myeloid leukemia. In: Advances in Cancer Therapy 4, Mediscript, London, U.K., pp. 5-16 (1993).